DNA, histones, and non-histone proteins make up chromatin. DNA that has been tightly wound around histones is essential for allowing DNA-binding proteins, including transcription factors, to be accessible for regulating particular gene expression. Investigating the epigenetic functions of transcription factors and histones is essential to learning more about the control of gene expression. The epigenetic method of chromatin immunoprecipitation sequencing (ChIP-seq) is a technique for mapping DNA-binding proteins and histone alteration throughout the body. Understanding the biological mechanisms linked to cancer requires assessing histone modification, investigating the DNA-protein interaction, and sequencing the target regions of DNA binding proteins.
